Download Pancreatic Cancer: Methods and Protocols (Methods in by Gloria H. Su PDF

By Gloria H. Su

This bold choice of crucial but novel tools for pancreatic melanoma study or melanoma learn often positive aspects a very good forged of authors who're esteemed leaders within the box. The authors offer a extensive diversity of tools for molecular, biochemical, pathological, and statistical research of sporadic and familial pancreatic melanoma, tools that may be utilized not just to easy, but additionally to translational pancreatic study. themes lined contain in vitro cellphone cultures, in vivo mouse versions, protein reports, mutation research, and therapy improvement.

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Extra resources for Pancreatic Cancer: Methods and Protocols (Methods in Molecular Medicine)

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Prepare a dissection station using a standard inverted microscope using a 30-gage needle on a syringe as the microdissecting tool (see Note 47). 2. 5% glycerol solution. 3. While viewing the tissue through the microscope, gently scrape the cell population of interest with the needle. The dissected cells will become detached from the 48 Bova et al. slide and form small dark clumps of tissue that can be collected on the needle by electrostatic attraction. Several small tissue fragments can be procured simultaneously.

Manual Microdissection of Tissue Sections on Glass Slides 1. Prepare stained sections 7–10 µm thick, on glass slides without cover slips. ” Prepare a dissection station using a standard inverted microscope using a 30-gage needle on a syringe as the microdissecting tool (see Note 47). 2. 5% glycerol solution. 3. While viewing the tissue through the microscope, gently scrape the cell population of interest with the needle. The dissected cells will become detached from the 48 Bova et al. slide and form small dark clumps of tissue that can be collected on the needle by electrostatic attraction.

5 M sucrose in PBS at 4°C. When tissue sinks, infusion is complete. Do not allow the infusion to go longer than 1 d (reduces quality of tissue morphology). 4. Place tissue samples in OCT or other embedding compound, and freeze rapidly. Section on a cryostat. 44 Bova et al. 4. 5. for comments. In terms of standard embedding materials, we have had good results with type VI paraffin (Richard Allan Scientific, Kalamazoo, MI), but many other products are available that are suitable. 5. 1. Frozen Sections (see Notes 29 and 30) 1.

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